Skip to main content
. Author manuscript; available in PMC: 2018 Oct 23.
Published in final edited form as: J Vis Exp. 2018 Apr 26;(134):10.3791/57498. doi: 10.3791/57498

Figure 2. Experimental protocol.

Figure 2.

(A) Schematic of the two-dimensional semi-denaturing detergent agarose gel electrophoresis (2D SDD-AGE). Begin by loading the sample in the right most lane, labeled in red. After termination of the first dimension run, rotate the gel 90° counter-clockwise. Run the second dimension electrophoresis. Solid arrow indicates the direction of the first dimension run, dotted arrow indicates the direction of the second dimension run. (B) Schema of transfer by capillary action.