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. Author manuscript; available in PMC: 2018 Oct 23.
Published in final edited form as: J Vis Exp. 2018 Apr 26;(134):10.3791/57498. doi: 10.3791/57498

Figure 4. Migration of MLKL-containing fibers during 1D and 2D SDD-AGE.

Figure 4.

(A) Whole cell lysates were harvested from cells stimulated with TSZ to undergo necroptosis. The lysates were subjected to SDD-AGE, and a Western blot was performed for MLKL. A characteristic smear was observed. (B) Whole cell lysates were harvested from cells stimulated with TSZ to undergo necroptosis. The lysates were subjected to 2D SDD-AGE and a Western blot was performed for MLKL. A sharp line at a 45° angle was observed, indicating that the fibers do not undergo dissociation during SDD-AGE.