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. Author manuscript; available in PMC: 2018 Jun 25.
Published in final edited form as: Bio Protoc. 2018 May 5;8(9):e2829. doi: 10.21769/BioProtoc.2829

Figure 1. LiCl purification allows amplification of cDNA from DSS-treated tissues.

Figure 1

Total RNAs were extracted from colonic tissues obtained from mice treated with DSS diluted at 3% in the drinking water or water controls. After cDNA synthesis, qPCR for the housekeeping gene 36B4 was performed and the product of amplification was visualized by electrophoresis. The presence of a smear indicates that the amplification of 36B4 cDNA was inhibited in DSS-treated mice before LiCl purification. After purification of the DSS- and non-DSS RNA samples with LiCl, clear bands indicate that 36B4 cDNA was successfully amplified from both DSS- and non-DSS samples.