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. Author manuscript; available in PMC: 2019 May 8.
Published in final edited form as: APL Bioeng. 2018 May 30;2(3):031904. doi: 10.1063/1.5026419

FIG. 3.

FIG. 3

Development of a 3D model for directed cell migration using collagen fiber alignment. (a) Randomly oriented 3D fibrillar collagen was used in the 3D random migration assay. (b) Representative image of the random architecture of collagen fibers imaged by confocal reflection microscopy. (c) Quantification of collagen fiber angles in (b) demonstrating a random distribution of the collagen fiber orientation. (d) Collagen mixed with magnetic beads and exposed to magnetic-induced flow results in 3D collagen with aligned fibers. (e) Aligned architecture of collagen fibers imaged by confocal reflection microscopy. (f) Distribution of collagen fiber angles in (e) shows a Gaussian distribution in the collagen fiber direction, centered at 90° relative to the magnet orientation. The scale bar is 100 μm.