FIGURE 5.
Three bulky nonpolar residues affect the threshold tensions of opening and closing. (a, b) Plot of channel number versus threshold tension for wild type MSL10 and the indicated variants. Data were corrected for access resistance. The data for wild-type MSL10 are the same in both plots. Error bars indicate standard deviation between the threshold pressure measured from 5 to 10 trials from a single patch containing the indicated number of channels. (c) The average open/close membrane tension ratios for MSL10 and variants including data from all patches shown in panels a and b. One sample for MSL10 F553L was left out as outlier. Error bars indicate standard deviation between the average open/close ratio for each patch