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. Author manuscript; available in PMC: 2009 Aug 25.
Published in final edited form as: J Neurosci. 2009 Feb 25;29(8):2534–2544. doi: 10.1523/JNEUROSCI.5865-08.2009

FIGURE 3. CXCL12 induces a time-dependent phosphorylation of the neuronal CXCR4 C-terminus.

FIGURE 3

Cortical neurons were treated with 20nM CXCL12 for the indicated time periods. Whole cell lysates were used for immunoblotting using a pERK, pAkt and pCXCR4 specific antibody (Ser 338/339). Receptor phosphorylation was blocked by the CXCR4 selective antagonist AMD3100 (100ng/ml); A) or by the PKC inhibitor Ro 31-8220 (100nM); B). The bar graph represents pCXCR4 band densities (mean ± S.E.M.) with respect to control levels and after normalization to actin band; C). (All CXCL12 treatments are significantly higher than control, i.e p<0.05; Ro 31-8220 treated groups are all significantly lower than respective CXCL12 alone, p<0.05)