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. Author manuscript; available in PMC: 2009 Aug 25.
Published in final edited form as: J Neurosci. 2009 Feb 25;29(8):2534–2544. doi: 10.1523/JNEUROSCI.5865-08.2009

FIGURE 4. μOR agonists inhibit CXCL12-induced phosphorylation of CXCR4.

FIGURE 4

Glia-free neuronal cultures were treated with 1μM morphine (or vehicle) or DAMGO for 24hr followed by 20nM CXCL12 for indicated amounts of time. Both agonists markedly reduce levels of pCXCR4 in total cellular extracts and CTAP (1μM) reversed this effect (A-C). The bar graphs (D) represent the mean ± S.E.M. of pCXCR4 band densities represented with respect to control levels from 3 independent identical experiments (all CXCL12 treatments are significantly higher than control, i.e. p<0.05 or less; DAMGO or MOR-treated groups are all significantly lower than corresponding CXCL12 alone, p<0.05 or less).