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. Author manuscript; available in PMC: 2019 Mar 13.
Published in final edited form as: J Vis Exp. 2018 Mar 13;(133):10.3791/57051. doi: 10.3791/57051

Figure 5. Singleplex and multiplex virus detection in urine using 300 nM probe.

Figure 5

(A) Zika detection in 10% urine in singleplex and multiplex format using 2.85 pfu of Zika (ZV) viral RNA with FAM-labeled probe (300 nM). (B) Chikungunya detection in 10% urine in singleplex and multiplex format using 242 copies of Chikungunya (CH) viral RNA with HEX-labeled probe (300 nM). (C) Dengue 1 detection in 10% urine in singleplex and multiplex format using 1.22 pfu of Dengue1 (D1) viral RNA with TAMRA-labeled probe (300 nM). (D) Visualization of RT-LAMP reactions through orange filter after excitation at 470 nm with blue LED light. As a positive control in urine experiments, a LAMP primer set targeting human mitochondrial DNA (mtDNA) was used with TET-labeled probe (300 nM). NTC = No template control on all panels. Reprinted with permission from Yaren et al. 201720.