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. Author manuscript; available in PMC: 2019 Mar 13.
Published in final edited form as: J Vis Exp. 2018 Mar 13;(133):10.3791/57051. doi: 10.3791/57051

Table 2. Viral titers in cell cultures and infected mosquito.

pfu: plaque-forming unit. Reprinted with permission from Yaren et al. 201720.

Virus, Strain (GenBank accession number) Family/Genus Viral titers
Zika virus (ZV), Puerto Rico (PRVABC59, KU501215.1) Flaviviridae/Flavivirus Group IV, positive, ssRNA 2.85 × 108 pfu/mL
Chikungunya virus (CH), British Virgin Togaviridae/Alphavirus Group 2.42 × 108
Islands (Asian lineage, KJ451624) IV, positive, ssRNA genomes/mL
Chikungunya virus (CH), La Reunion (Indian Ocean lineage, LR2006-OPY1, KT449801) Togaviridae/Alphavirus Group IV, positive, ssRNA 1.89 × 108 genomes/mL
Chikungunya virus (CH), La Reunion extracted total NA from Aedes aegypti female (Indian Ocean lineage, LR2006- OPY1, KT449801) Togaviridae/Alphavirus Group IV, positive, ssRNA 3.85 × 105 genomes/mL
Dengue serotype 1 (D-l), Key West (FL) (JQ675358) Flaviviridae/Flavivirus Group IV, positive, ssRNA 1.22 × 106 pfu/mL

Zika (ZV) Mosquito identity, Strain Leg titer pfu/mL

ZV 9, Puerto Rico 4.76 × 103