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. Author manuscript; available in PMC: 2018 Aug 22.
Published in final edited form as: J Immunol. 2018 Jul 18;201(5):1389–1399. doi: 10.4049/jimmunol.1800324

Figure 2. PMN co-culture does not affect cytokine production in stimulated T cells.

Figure 2

(A and B) PBMC stimulated with or without anti-CD3/CD28 mAbs were co-cultured with increasing numbers of PMN for 16 hours, and the percentage of T cells producing cytokines was determined using intracellular staining of flow cytometry. Live CD8 and CD4 T cells were gated. Results are shown as mean ± SEM (n =3) from three different donors. P =NS (not significant). (C and D) Dotplots show a representative data of three different donors as shown in the cumulative data graph (top panels). Fixed PMN were used.