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. Author manuscript; available in PMC: 2018 Aug 22.
Published in final edited form as: J Immunol. 2018 Jul 18;201(5):1389–1399. doi: 10.4049/jimmunol.1800324

Figure 4. PMN-mediated T cell inhibition is prevented by anti-P3 blocking antibody and requires P3 enzyme activity.

Figure 4

CFSE-labeled PBMC stimulated with anti-CD3/CD28 mAbs were co-cultured with PMN at the indicated PMN: PBMC ratio. (A) PMN were treated with anti-P3 mAb (clone WGM2) or mIgG (clone 11711). The PMN to PBMC ratio is 3:1. Results from five different donors (n =5) are shown as mean ± SEM. (B) PMN were pre-incubated with or without PMSF, Elafin or α1-Antitrypsin (AAT) before adding to PBMC cultures. The PMN to PBMC ratio is 4:1. Results shown as mean ± SEM are representative of three different donors performed in triplicates. ****P < 0.0001, ***P < 0.0005, **P < 0.005, *P < 0.05. P =NS (not significant). Fixed PMN were used.