R1479, 2′F-4′N3-C, and 2′diF-4′N3-C inhibit Sudan virus (SUDV). (a) Representative dose-response curves for R1479 (green circles), 2′F-4′N3-C (blue triangles), and 2′diF-4′N3-C (red squares) against SUDV (Gulu variant) using a focus forming unit (FFU) assay. FFU assays for R1479 and 2′F-4′N3-C were performed once, and was repeated once for 2′diF-4′N3-C. (b) Reduction of infectious SUDV yield (in log scale) by R1479, 2′diF-4′N3-C, and 2′F-4′N3-C. TCID50/mL values (in log scale) for each data point represent the mean of quadruplicate infections for each concentration in the 10-point, 3-fold dilution series for each compound performed once.