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. Author manuscript; available in PMC: 2018 Aug 13.
Published in final edited form as: Bio Protoc. 2018 Jul 5;8(13):e2919. doi: 10.21769/BioProtoc.2919

Figure 2. Fluorescence images of a 4% labeled collagen.

Figure 2

A. Single confocal slice (XY) and a 10 μm YZ projection (right) of a 4% atto 488-labeled 3 mg/ml rat-tail collagen gel polymerized at 21 °C. B. 3D rendering of the collagen gel shown in panel A (X, Y, Z dimensions: 100 × 100 × 80 μm, respectively). C. High-resolution max intensity projections of an HT-1080 fibrosarcoma cancer cell transfected with EGFP-α-actinin (magenta) migrating through Atto 565-labeled 3 mg/ml rat-tail collagen (green) polymerized at 16 °C. Images show first frame (left) and 63rd frame (right) in a time-series (imaged every 0.5 μm in Z over 15 microns, every 30 sec). Time is in minutes.