Fig. 8.

Nav1.2 expressions at AISs of excitatory neurons in neocortex and hippocampus. Immunofluorescence histochemistry of P15.5 wild-type neocortices and hippocampi stained with anti-Nav1.2 (G-20, magenta), anti-ankyrin G (green), and anti-Tbr1 (cyan) antibodies, and counterstained with 4′−6-diamidino-2-phenylindole (DAPI, gray) and their merged images. Arrows indicate Nav1.2 and ankyrin G-double immunoreactive AISs of Tbr1-expressing neocortical pyramidal cells. Arrowheads indicate Nav1.2 and ankyrin G-double immunoreactive AISs of hippocampal pyramidal cells. Representative images of four or more slices are shown. o stratum oriens, p stratum pyramidale. Scale bars: 20 μm