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. Author manuscript; available in PMC: 2019 Apr 25.
Published in final edited form as: Sci Transl Med. 2018 Apr 25;10(438):eaal1803. doi: 10.1126/scitranslmed.aal1803

Fig. 2. Bio-AMS resistance.

Fig. 2.

(A) Frequency of spontaneous resistance with Bio-AMS at concentrations of 1×, 25× and 50× the MIC. (B) Overexpression of Rv3406 causes resistance to Bio-AMS. H37Rv either contained the vector control (pTE-mcs) or the overexpression plasmid pGMEH-Ptb38-rv3406. One spontaneously resistant isolate (Bio-AMS-5R1) was included as a control. (C) Saturation curve used to determine the kinetic parameters for Bio-AMS oxidation by Rv3406. Data were fitted by non-linear regression to the Michaelis-Menten equation (left panel). Time course for Rv3406 catalyzed formation of UV active metabolite 3 as monitored by HPLC (right panel).