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. Author manuscript; available in PMC: 2019 Sep 27.
Published in final edited form as: J Innate Immun. 2018 Nov 8;11(1):99–108. doi: 10.1159/000494070

Figure 1. LPS treatment altered macrophage differentiation.

Figure 1.

(A). Freshly isolated bone marrow precursors expressed the macrophage markers CSF1R, F4/80, and CD11b after 3 d culture in L929 conditioned media. (B). Adding LPS to the media (250 ng/ml for 16 h) on day 0 had no effect on CSF1R or F4/80 expression. However treating bone marrow precursors with LPS after 2 d (middle) or 7 d (right) decreased CSF1R (top panels) and increased F4/80 expression (bottom panels). Representative FACS data shown. (C). MFI data from multiple 2 d experiments showed that LPS reduced CSF1R (**P < 0.005, n = 11) and increased F4/80 (**P < 0.005, n = 11). (D). Each separate bone marrow isolette displayed similar trends in F4/80 and CSF1R expression following LPS treatment on day 2. (E). LPS increased mRNA expression of Adgre1 (gene encoding F4/80) and the scavenger receptor Msr1 (CD204) (***P < 0.001, n = 10).