Figure 3. Effect of MAPK inhibition on LPS-induced changes in BMDM differentiation.
(A). FACS of BMDM treated with LPS or LPS following 1 h pretreatment with a MAPK inhibitor cocktail. MAPK inhibition prevented the LPS-induced reduction in CSF1R expression (*P < 0.05, n = 5). However F4/80 expression was not affected. (B,C). Representative FACS experiment showing effects of LPS +/−MAPK inhibition on F4/80 and CSF1R expression in BMDM. MAPK inhibition prevented the LPS-induced changes in the percentage of F4/80+ cells also expressing CSF1R. Each line in (C) represents an independent experiment.