Table 1.
Properties of human meizoIIRRΔF1 compared to thrombin
| Fibrinogena | PAR1a | Protein Ca,b | FPRc | FPRd | Na+e | rf | |
|---|---|---|---|---|---|---|---|
| Thrombin | 17±1 | 39±1 | 0.22±0.01 | 88±3 | 3.2±0.2 | 160±20 | 0.72±0.07 |
| MeizoIIRRΔF1 | 0.23±0.04 | 0.75±0.08 | 0.044±0.02 | 91±2 | 7.4±0.1 | 91±9 | 0.83±0.07 |
All values are kcat/Km in μM-1 s-1, except Na+ and r.
a
5 mM Tris, 145 mM NaCl, 0.1 % PEG, pH 7.4, 37 °C.
b
In the presence of 5 mM CaCl2 and 100 nM thrombomodulin.
c
5 mM Tris, 200 mM NaCl, 0.1 % PEG, pH 8.0, 25 °C.
d
5 mM Tris, 200 mM ChCl, 0.1 % PEG, pH 8.0, 25 °C.
e
Values refer to the intrinsic equilibrium association constant, KA, in M-1 or the ratio r = [E*]/[E] (see eq. 1) determined by stopped-flow measurements under conditions of 5 mM Tris, 0.1 % PEG, pH 8.0, 15 °C.
f
Values refer to the intrinsic equilibrium association constant, KA, in M-1 or the ratio r = [E*]/[E] (see eq. 1) determined by stopped-flow measurements under conditions of 5 mM Tris, 0.1 % PEG, pH 8.0, 15 °C.