Table 6.

Summary of Myosin or Heart Cross-reactive T Cell Epitopes of Streptococcal M5 Protein^¥

Peptide	Sequence£	Origin of T Cell Clone or Response
1–25	§TVTRGTISDPQRAKEALDKYELENH	ARF/Valve(100)
81–96	§DKLKOORDTLSTOKETLEREVON	ARF/Valve(100)
163–177	§ETIGTLKKILDETVK	ARF/Valve(100)
337–356	LRRDLDASREAKKOVEKAL	Normal/PBL(133)
347–366	AKKQVEKALEEANSKLAALE	Mice (132)/Normal PBL(133)
397–416	LKEOLAKOAEELAKLRAGKA	ARF/PBL(133)
NT4 40–58	GLKTENEGLKTENEGLKTE	BALB/c/Lymph node*
NT5 59–76	KKEHEAENDKLKQQRDTL
B1B2 137–154	VKDKIAKEQENKETIGTL
B2 150–167	TIGTLKKILDETVKDKIA
C2A 254–271	EASRKGLRRDLDASREAK
C3 293–308	KGLRRDLDASREAKKQ

^¥Taken from reference 27 with permission from ASM press

^§Amino terminal TVTRGTIS sequence was taken from the M5 amino acid sequence published by Manjula et al (206) and deviates from the M5 sequence published by Miller et al (207) at positions 1 and 8. Other marked(§) sequences (81–96) and (163–177) were taken from the PepM5 sequence as reported by Manjula (206). These two sequences are found in the sequence as 67–89 and 174–188, respectively, as reported by Miller et al (207). All other sequences shown above are from the M5 gene sequence reported by Miller et al (207). Underlined sequences are those shared among the epitopes/sequences compared in Table 4.

^#PBL, peripheral blood lymphocytes, ARF, acute rheumatic fever

^*BALB/c mice immunized with purified human cardiac myosin and the recovered lymph node lymphocytes were stimulated with each of the peptides in tritiated thymidine uptake assays.