Table 6.
Peptide | Sequence£ | Origin of T Cell Clone or Response |
---|---|---|
1–25 | §TVTRGTISDPQRAKEALDKYELENH | ARF/Valve(100) |
81–96 | §DKLKOORDTLSTOKETLEREVON | ARF/Valve(100) |
163–177 | §ETIGTLKKILDETVK | ARF/Valve(100) |
337–356 | LRRDLDASREAKKOVEKAL | Normal/PBL(133) |
347–366 | AKKQVEKALEEANSKLAALE | Mice (132)/Normal PBL(133) |
397–416 | LKEOLAKOAEELAKLRAGKA | ARF/PBL(133) |
NT4 40–58 | GLKTENEGLKTENEGLKTE | BALB/c/Lymph node* |
NT5 59–76 | KKEHEAENDKLKQQRDTL | |
B1B2 137–154 | VKDKIAKEQENKETIGTL | |
B2 150–167 | TIGTLKKILDETVKDKIA | |
C2A 254–271 | EASRKGLRRDLDASREAK | |
C3 293–308 | KGLRRDLDASREAKKQ |
Taken from reference 27 with permission from ASM press
Amino terminal TVTRGTIS sequence was taken from the M5 amino acid sequence published by Manjula et al (206) and deviates from the M5 sequence published by Miller et al (207) at positions 1 and 8. Other marked(§) sequences (81–96) and (163–177) were taken from the PepM5 sequence as reported by Manjula (206). These two sequences are found in the sequence as 67–89 and 174–188, respectively, as reported by Miller et al (207). All other sequences shown above are from the M5 gene sequence reported by Miller et al (207). Underlined sequences are those shared among the epitopes/sequences compared in Table 4.
PBL, peripheral blood lymphocytes, ARF, acute rheumatic fever
BALB/c mice immunized with purified human cardiac myosin and the recovered lymph node lymphocytes were stimulated with each of the peptides in tritiated thymidine uptake assays.