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. Author manuscript; available in PMC: 2018 Nov 28.
Published in final edited form as: Bio Protoc. 2018 Oct 20;8(20):e3061. doi: 10.21769/BioProtoc.3061

Table 1.

Chronic Mild Stressors (from 4–7 weeks)

WEEK 1 DAY 1 DAY 2 DAY 3 DAY 4 DAY 5 DAY 6 DAY 7
8:30 am Water
deprivation
Damp
Bedding
Tube
Restraint
Water
Deprivation
Damp
Bedding
Tube
Restraint
Water
Deprivation
5:30 pm Tail
Suspension
Food
Deprivation
Strobe
Light
Tail
Suspension
Food
Deprivation
Strobe
Light
Anhedonia
Test
WEEK 2 DAY 8 DAY 9 DAY 10 DAY 11 DAY 12 DAY 13 DAY 14
8:30 am Tail
Suspension
Tube
Restraint
Water
Deprivation
Damp
Bedding
Tube
Restraint
Water
Deprivation
Damp
Bedding
5:30 pm Food
Deprivation
Strobe Light Tail
Suspension
Food
Deprivation
Strobe Light Tail
Suspension
Anhedonia
Test
WEEK 3 DAY 15 DAY 16 DAY 17 DAY 18 DAY 19 DAY 20 DAY 21
8:30 am Food
Deprivation
Water
Deprivation
Damp
Bedding
Damp
Bedding
Empty
Bottle
Water
Deprivation
Damp
Bedding
5:30 pm Strobe
Light
Tail
Suspension
Tube
Restraint
Food
Deprivation
Strobe
Light
Tail
Suspension
Anhedonia
Test
WEEK 4 DAY 22 DAY 23 DAY 24 DAY 25 DAY 26 DAY 27 DAY 28
8:30 am Tail
Suspension
Tube
Restraint
Water
Deprivation
Damp
Bedding
Tube
Restraint
Water
Deprivation
Anhedonia
Test
5:30 pm Food
Deprivation
Strobe
Light
Tail
Suspension
Food
Deprivation
Strobe
Light
Tail
Suspension
Sacrifice