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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: FASEB Bioadv. 2018 Nov 14;1(3):167–179. doi: 10.1096/fba.2018-00015

Figure 2: IGFBP-4 decreases ECM production levels ex vivo in human lung and skin organ culture.

Figure 2:

A. IGFBP-4 decreases baseline and TGF-ß induced collagen mRNA levels in human lung. Normal human donor lung tissues were cultured with 2 μg/ml of rhIGFBP-4 with or without 10ng/ml TGF-ß1 for 4 days. Collagen mRNA expression was analyzed by real time PCR. The graph shows results from lung tissues of three different donors. B. IGFBP-4 decreases baseline and TGF-ß induced collagen levels in human lung. Donor lungs were cultured as in A, except that the culture time was extended to 7 days. Lung tissues were homogenized and equal amounts of total protein analyzed by WB. The graph shows results from lung tissues of three different donors. C. IGFBP-4 decreases TGF-ß-induced hydroxyproline levels in human skin. Human newborn foreskin was maintained in organ culture in the presence of 2 μg/ml rhIGFBP-4 with or without 10 ng/ml TGF-ß1 for 7 days. Collagen content was measured using hydroxyproline assay. The graph includes results from the skin of 4 different donors. * p < 0.05, ** p < 0.01.