Figure 2.

Expression of miR-21 and miR-181b is lost in Gr1⁺CD11b⁺ cells from late, but not early, septic NFI-A knockout mice. Bone marrow Gr1⁺CD11b⁺ cells were isolated from sham and septic mice. (a) miRNA-enriched RNA was isolated, and levels of miR-21 and miR-181b were measured by RT-qPCR using miR-21 and miR-181b specific assay primer sets. Values were normalized to U6 RNA as an internal control. Data are expressed as mean ± SD (*P < 0.05) of five mice per group. Values from sepsis mice are presented relative to sham values (set at onefold) and represent one of two experiments. *Compared with sham. cKO: conditional knockout. (b) Knockout of NFI-A does not affect Stat3 phosphorylation or C/EBPβ protein levels in Gr1⁺CD11b⁺ cells during sepsis. Whole protein extracts from Gr1⁺CD11b⁺ cells were subjected to Western blotting of the indicated proteins. Lower panels show densitometry of the Stat3 and C/EBPb protein bands. Values were normalized to β-actin and are presented relative to sham. The results represent one of two experiments.