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. Author manuscript; available in PMC: 2018 Dec 11.
Published in final edited form as: Bio Protoc. 2018 Nov 5;8(21):e3068. doi: 10.21769/BioProtoc.3068

Figure 1. Lightly confluent LLC-MK2 cells.

Figure 1.

Once overlaid agar is solidified, cells can no longer proliferate. Therefore, the cells want to be confluent when you overlay agar on the cells (Scale bar = 100 μm).