Table 1.
Patient | Age | Gene | Protein | Nucleotide affected | Amino acid change | Somatic/germline | LOH | Evidence for pathogenicity |
---|---|---|---|---|---|---|---|---|
1 | 82 | CDKN1A | p21 | c.350G>A | Cys117Tyr | Germline | No | genetic |
2 | 64 | CDKN1A | p21 | c.26G>A | Arg9His | ND | No | Cytoplasmic accumulation/abnormal migration and genetic |
3 | 69 | CDKN2B | p15 | c.256G>A | Asp86Asn | Germline | Yesa | Impaired CDK6 binding and genetic |
4 | 60 | CDKN2C | p18 | c.62 T>A | Leu21His | Somatic | No | Impaired CDK6 binding, abnormal migration and genetic |
5 | 53 | CDKN2C | p18 | c.494C>T | Thr165Ile | Germline | Yesb | genetic |
LOH within and surrounding the CDKN2B gene was established using microsatellite markers D9S157, D9S171, and D9S1853 and direct DNA sequencing
LOH within and surrounding the CDKN2C gene was established using microsatellite markers D1S507 and D1S2664, and direct DNA sequencing