Fig. 8.

MoPdeH promotes the enzymatic activity of MoImd4. (A) Enzymatic activity of purified His-MoImd4 proteins, with 1 T and 2 T of the purified GST-MoPdeH protein. The production of XMP was monitored by the absorbance at 290 nm. “T” represents the total quantity of the His-MoImd4 protein. Experiments were repeated three times with similar results. The error bars indicate standard deviation of three replicates. Asterisk indicates statistically significant differences (Duncan’s new multiple range test, p<0.01). (B) In vivo, we extracted the total protein from the wild type Guy11 and ΔMopdeH as the crude enzyme of MoImd4, then added them into the enzymatic reaction system for 5 min, the production of XMP was monitored by the absorbance at 290 nm. Experiments were repeated three times with similar results. The error bars indicate standard deviation of three replicates. Asterisk indicates statistically significant differences (Duncan’s new multiple range test, p<0.01).