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. Author manuscript; available in PMC: 2019 Sep 16.
Published in final edited form as: Mol Plant Pathol. 2019 Jan 11;20(4):500–518. doi: 10.1111/mpp.12770

Table 3.

Phenotype analysis of the wild type, ΔMoimd4 mutant, the complement strains, point mutation mutants and three CBS domain deletion mutants.

Strain Colony diameter (CM)a Colony diameter (MM)a Colony diameter (MM + XMP)b Conidiation (x100/cm2)c Conidiation+XMP
(x100/cm2)d
Guy11 4.8 ± 0.1A 2.9 ± 0.1A 3.0 ± 0.2A 741.9 ± 50.9A 746.3 ± 73.1A
ΔMoimd4 3.1 ± 0.1EF 0.9 ± 0.1D 2.8 ± 0.1A 27.9 ± 2.5C 25.1 ± 4.1C
T268AR269A 3.0 ± 0.1FG 1.3 ± 0.1C 2.3 ± 0.3B 29.3 ± 6.4C 30.7 ± 3.8C
S345AC347A 2.0 ± 0.1H   1.4 ± 0.1B 2.7 ± 0.2A 46.3 ± 7.3C 55.1 ± 5.0C
D380AG382A 3.3 ± 0.2E 0.8 ± 0.1D 1.3 ± 0.1C 41.9 ± 4.5C 33.1 ± 8.2C
G403A 3.2 ± 0.1EF 0.8 ± 0.1D 0.8 ± 0.1D 46.8 ± 4.1C 43.9 ± 7.6C
R458AY459A 3.9 ± 0.1C 0.4 ± 0.1E 0.4 ± 0.1E 33.0 ± 3.6C 27.9 ± 6.1C
Y428A 3.7 ± 0.1D 0.3 ± 0.1E 0.5 ± 0.1DE 21.6 ± 3.8C 21.8 ± 5.4C
ΔCBS1 4.5 ± 0.2B 3.0 ± 0.2A 3.0 ± 0.1A 684.2 ± 40.1AB 624.5 ± 50.2B
ΔCBS2 4.5 ± 0.1B 2.9 ± 0.2A 3.0 ± 0.2A 624.3 ± 32.2B 630.2 ± 35.1B
ΔCBS1CBS2 4.1 ± 0.1C 0.4 ± 0.1E 0.4 ± 0.1E 33.7 ± 2.1C 29.9 ± 6.5C
MoImd4 4.8 ± 0.2A 2.9 ± 0.1A 2.9 ± 0.2A 726.3 ± 32.2A 746.3 ± 15.0A
a.

Colony diameter of the indicated strains on CM or MM media after 7 days incubation at 28°C; ±SD was calculated from three repeated experiments and letters indicate statistically significant differences (Duncan's new multiple range test, letters mean p<0.01).

b.

Colony diameter of the indicated strains on MM media which added 1mM XMP after 7 days incubation at 28°C; ±SD was calculated from three repeated experiments and letters indicate statistically significant differences (Duncan's new multiple range test, letters mean p<0.01).

c.

Quantification of the conidial production of the indicated strains from SDC cultures in the dark for 7 d, followed by incubation under constant illumination for 3 d at room temperature; ±SD was calculated from three repeated experiments and letters indicate statistically significant differences (Duncan's new multiple range test, letters mean p<0.01).

d.

Quantification of the conidial production of the indicated strains from SDC cultures which added 1mM XMP in conidial suspension in the dark for 7 d, followed by incubation under constant illumination for 3 days at room temperature; ±SD was calculated from three repeated experiments and letters indicate statistically significant differences (Duncan's new multiple range test, letters mean p<0.01).