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. Author manuscript; available in PMC: 2007 Dec 21.
Published in final edited form as: Virology. 2006 Nov 13;360(1):172–181. doi: 10.1016/j.virol.2006.10.012

Fig. 2.

Fig. 2

Northern blot analysis of expression of late transcripts in undifferentiated (U) and differentiated (D) W12 cells. Radiolabelled probes were either the insert from plasmid pRL1 containing a portion of the L1 gene (nts 6151–6818) or the insert from plasmid pE4 containing the entire E4 gene sequence (nts 3339–3598) (E4). Total; total cellular RNA. poly(A)+; polyadenylated RNA. Blots were hybridised in 50% formamide 5× SSC at 42 °C for 16 h and washed to 0.1× SSC at 65 °C. Blots were stripped and reprobed with radiolabelled actin or GAPDH sequences as loading controls.