5′RACE |
|
L1 6069 (RT) |
CTCTATTATCCACACCTGCAT |
LI 5968 |
TGACCACGACCTACCTCAACACCTACACA |
L1 5776 |
TGTCCAACTGCAAGTAGTCTGGATGTTCCT |
LI 5690 |
TACTGGGACAGGAGGCAAGTAGACAGTGGCCTCAC |
L2 4506 |
GGTCTTACAGGAGCAAGTGTATCTGTAGCT |
L2 4456 |
TGGAATATACCCAGTGCGTCCGCCTGTA |
E5 3890 |
GCACGCCAGTAATGTTGTGGATGC |
E2 3845 |
GACATAAATCCAGTAGACACTGTAAT |
E4 3596 |
CAGTTAATCCGTCCTTTGTGTGAGCT |
E4 3451 |
CTTCGGTGCCCAAGGCGACGGCTTTGGTAT |
|
|
3′RACE |
|
Outer primer 7004 |
CTGCAGACCTAGATCAGTTTC |
Inner primer 7047 |
CAAGCAGGATTGAAGGCC |
Primer X 5746 |
CCAGACTACTTGCAGTTGGAC |
Primer Y 5948 |
TGTTGAGGTAGGTCGTGGTCAGC |
|
|
Primer extension |
|
E6 222 |
CGTCGCAGTAACTGTTGCTTGCAGTACAC |
E7 766 |
CGCACAACCGAAGCGTAGAGTCACAC |
E1 979 |
CGTTCTCGTCATCTGATATAGCATCCC |
E4 3412 |
CGGGGTGGTTGGCCAAGTGCTGCC |
E5 4126 |
GTAACAATTACATTATGTACATATACATTATG |