Table 1. A list of 46 enzymes examined in O. viverrini by MEE under different experimental conditions, including the number of bands of activity exhibited per enzyme following staining.
a A = 0.01 M Citrate-phosphate pH 6.4, B = 0.02 M Phosphate pH 7.0, C = 0.05 M Tris-maleate pH 7.8.
b Enzymes showed one broad band. The following enzymes showed no activity following electrophoresis and staining: alcohol dehydrogenase (ADH, 1.1.1.1), aldehyde dehydrogenase (ALDH, 1.2.1.5), alkaline phosphatase (AP, 3.1.3.1), diaphorase (DIA, 1.6.*.*), glucose dehydrogenase (GLDH, 1.1.1.47), glycollate oxidase (GOX, 1.1.3.1), glycerol-3-phosphate dehydrogenase (GPD, 1.1.1.8), leucine aminotransferase (LAP, 3.4.11.1), mannose-phosphate isomerase (MPI, 5.3.1.8), phosphoglycerate kinase (PGK, 2.7.2.3), superoxide dismutase (SOD, 1.15.1.1), sorbital dehydrogenase (SORDH, 1.1.1.14) and xanthine oxidase (XO, 1.2.3.2).
| Enzymes (abbreviation, enzyme commission no.) | Running buffera | Running time (h) |
|---|---|---|
| Enzymes (one band) | ||
| Acid phosphatase (ACP, 3.1.3.2) | B | 2.30 |
| Aldolase (ALD, 4.1.2.13) | B | 2.00 |
| Esterase (EST, 3.1.1.1) | B | 2.00 |
| Fructose-1,6-diphosphatase (FDP, 3.1.3.11)b | B | 2.30 |
| Glucose-6-phosphate dehydrogenase (G6PD, 1.1.1.49)b | B, C | 2.30 |
| Glucose-phosphate isomerase (GPI, 5.3.1.9) | B, C | 2.30 |
| β-Hydroxybutyrate dehydrogenase (HBDH, 1.1.1.30) | B | 1.30 |
| Isocitrate dehydrogenase (IDH, 1.1.1.42)b | A, B | 2.30 |
| Nucleoside phosphorylase (NP, 2.4.2.1) | B | 1.30 |
| Peptidase leucine-glycine-glycine (PEP-B, 3.4.11.4) | B | 2.30 |
| Peptidase lysine-leucine (PEP-C, 3.4.13) | B | 2.30 |
| Peptidase phenylalanine-proline (PEP-D, 3.4.13) | B, C | 2.00 |
| Phosphoglycerate mutase (PGAM, 2.7.5.3)b | C | 2.30 |
| 6-Phosphogluconate dehydrogenase (6PGD, 1.1.1.44) | B | 1.30 |
| Pyruvate kinase (PK, 2.7.1.40)b | C | 2.30 |
| Uridine monophosphate kinase (UMPK, 2.7.1.48) | C | 2.30 |
| Enzyme (two bands) | ||
| Creatine kinase (CK, 2.7.3.2) | B, C | 2.30 |
| Fumarate hydratase (FUM, 4.2.1.2) | B | 2.30 |
| Glutamate dehydrogenase (GDH, 1.4.1.3) | B | 1.30 |
| Lactate dehydrogenase (LDH, 1.1.1.27) | B | 1.30 |
| Malic enzyme (ME, 1.1.1.40) | B, C | 2.30 |
| Purine peptidase valine-leucine (PEP-A, 3.4.13.11) | B, C | 2.00 |
| Enzymes (three bands) | ||
| Aconitate hydratase (ACON, 4.2.1.3) | B | 1.30 |
| Adenylate kinase (AK, 2.7.4.3) | B | 2.30 |
| Alanine aminotransferase (GPT, 2.6.1.2) | B | 2.00 |
| Aspartate aminotransferase (GOT, 2.6.1.1) | B | 2.00 |
| Enolase (ENOL, 4.2.1.11) | A, B | 2.00 |
| Hexokinase (HK, 2.7.1.1) | B, C | 2.30 |
| Malate dehydrogenase (MDH, 1.1.1.37) | B, C | 2.30 |
| Phosphoglucomutase (PGM, 2.7.5.1) | B, C | 2.30 |
| Enzyme (four bands) | ||
| Nucleotide diphosphate kinase (NDPK, 2.7.4.6) | B | 2.30 |
| Enzymes (five bands) | ||
| Glyceraldehyde-3-phosphate dehydrogenase (GAPD, 1.2.1.12) | B | 2.30 |
| Triose-phosphate isomerase (TPI, 5.3.1.1) | B | 2.00 |