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. Author manuscript; available in PMC: 2009 Feb 3.
Published in final edited form as: Protein Expr Purif. 2007 Oct 24;57(2):206–216. doi: 10.1016/j.pep.2007.10.013

Fig. 7.

Fig. 7

Pichia pastoris-produced CD81 binds Hepatitis C virus E2. Increasing concentrations of hCD81, bacteriorhodopsin and bacterially-produced hCD81 EC2 were bound to an ELISA plate and tested for reactivity with a saturating concentration of anti-CD81 M38. Data are expressed as optical density at 450 nm (A). A saturating concentration of P. pastoris hCD81, control bacteriorhodopsin protein and hCD81 EC2 were bound to an ELISA plate and tested for their ability to bind increasing amounts of soluble HCV E2. Bound E2 was realized with an anti-E2 mAb, 9/27 and anti-rat Ig-HRP. Data are expressed as optical density at 450 nm (B).