Table 1.
Proteins identified in vernix by MALDI MS, N- and C-terminal sequence analyses and peptide mass fingerprinting
| Peak no.† |
Identified protein | Swissprot accession number |
MALDI determined mass/ theoretical mass |
N-terminal sequence | C-terminal sequence |
MALDI sequence coverage (%) |
MALDI matching fragments (no.) |
|---|---|---|---|---|---|---|---|
| 1 | Ubiquitin (1-76) | P62991 | 8568 / 8565 | MQIFVKTLTGKTITL... | NA | 64 | 5 |
| 1 | Ubiquitin (1-72) | P62991 | 8183 / 8181 | MQIFVKTLTGKTITL... | NA | NA | NA |
| 1 | Ubiquitin (1-70) | P62991 | 7916 / 7912 | MQIFVKTLTGKTITL... | ...HLV | NA | NA |
| 3 | Psoriasin | P31151 | 11374 / 11367 | DNTQAERSIIGMIDM... | NA | 63 | 8 |
| 3 | Lymphocyte antigen Ly6E | Q16553 | NA | LMXFSXLNQKSNLY... | NA | NA | NA |
| 4 | Lysozyme C | P61626 | NA | KVFERXELARTLKRL... | NA | 59 | 8 |
| 4 | Transthyretin | P02766 | NA | GPTGTGESKAPLMV... | NA | NA | NA |
| 4 | Apolipoprotein C-1 (29-83) | P02654 | 6433 / 6432 | DVSSALDKLKEFGNT... | NA | NA | NA |
| 5 | Calgranulin C | P80511 | NA | XXLEEHLEGIVNIFHQ... | NA | NA | NA |
| 5 | Squamous cell carcinoma antigen 1 and/or 2(SCCA 1/2) (5-)* |
P29508/ P48594 |
NA | SCANTKFMFDLFQQF... | NA | NA | NA |
| 6 | Apolipoprotein A-II (38- 101) |
P02652 | 7145 / 7145 | FQTVTDYGKDLMEKV... | NA | NA | NA |
| 6 | Haemoglobin α-chain | P69905 | 15135 / 15126 | VLSPADKTNVKAAWG... | ...KIR | 74 | 6 |
| 6 | Haemoglobin β-chain | P68871 | 15871 / 15867 | VHLTPEEKSAVATLW... | NA | 75 | 8 |
| 7 | Profilaggrin (1-)* | Q01720 | NA | XTLLENIFAIINLFK... | NA | NA | NA |
| 7 | Calgranulin A | P05109 | NA | MLTELEKALNSIIDVYHKY... | NA | 65 | 6 |
| 8 | UGRP-2 | Q96QR1 | NA | FLVGSAKPVAQPVAA... | NA | NA | NA |
| 9 | Hypothetical protein FLJ40379 |
Q8N7T4 | NA | VTXEVXMNVVQKLDH... | NA | NA | NA |
| 10 | UGRP-1 (22-93) | Q96PL1 | NA | FLINKVPLPVDKLAP... | ...LSHLV | 94 | 8 |
| 10 | UGRP-1 (34-93) | Q96PL1 | NA | LAPLPLDNILPFMD... | ...LSHLV | 93 | 6 |
| 10 | UGRP-1 (35-93) | Q96PL1 | NA | APXPLDNILPFMDPL... | ...LSHLV | 93 | 6 |
| 10 | UGRP-1 (40-93) | Q96PL1 | NA | DNILPFMDPLKLLLK... | ...LSHLV | 93 | 6 |
| NA | Cystatin A | P01040 | NA | IPGGLSEAKPATPEIQEIVD... | NA | 73 | 6 |
| NA | Calgranulin B | P06702 | NA | NA | NA | 80 | 9 |
| NA | Capase 14 precursor / C- terminal fragment* |
P31944 | NA | NA | NA | 37/86 | 9/8 |
| NA | Haemoglobin γ 1 / 2 chain | P69891/P69892 | NA | NA | NA | 62 | 7 |
†
Peak numbers correspond to peaks in fig. 2
*
fragment of unknown size, NA, not applicable