Table 2. Effects of compensatory mutations on the infectivity of SFV4 genome.
Alteration in the palmitoylation site of nsP1 |
Compensatory mutation introduced |
Full-length RNA infectivity (p.f.u. μg−1)* |
---|---|---|
None | None | 9.0 × 105 |
None | P181Q | 7.8 × 105 |
None | L234F | 1.3 × 106 |
None | Q357L | 1.2 × 106 |
None | M124V + A197D | 4.4 × 105 |
None | ΔG224 + T352S | 2.0 × 105 |
ΔCCC (418–420) | L234F | 5.7 × 105 |
Δ RSLTCCC (414–420) | L234F | 1.0 × 103 |
Infectivity of full-length SFV genomes was measured by infectious centre assay. The numbers shown represent data from one of two experiments with similar results.