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Measurement of NAADP levels in pancreatic acinar cell samples. (A) Schematic to show the preparation of pancreatic acinar cell samples. These samples were then diluted for use in the assay. (B) A competitive displacement curve was generated using standard concentrations of NAADP (black filled circles). Dilutions of the pancreatic acinar cell sample were determined from this curve and are shown on the trace (red triangles).
