Figure 5. Heterologous expression of Opn4L and Opn4S suggests that both variants can form a sensory photopigment.
Representative whole cell patch-clamp recording from Neuro-2A cells transfected with Opn4L (A) or Opn4S (B), in the presence of 9-cis-retinal. Monochromatic light stimuli 420nm and 480nm (presented for 10s 8 × 1014 photons cm−2 s−1) evoke a stimulus dependent inward current. Holding potential −50mV. No differences in amplitude of responses, kinetics or spectral sensitivity were observed between Opn4L and Opn4S.