Table 1.
| Gene | Abbrev | Genbank | Forward primer | Reverse primer | Reference to mRNA localization* |
|---|---|---|---|---|---|
| Sertoli cell | |||||
| Anti-Mullerian hormone | Amh | nm_007445 | tcctacatctggctgaagtgatatggg | aggttctgtgtgccccgcag | Munsterberg & Lovell-Badge (1991) |
| Aquaporin 8 | Aqp8 | nm_007474 | gctggcagtcacagtgatcgga | cctggacgatggcaaaggctg | Badran & Hermo (2002) |
| Claudin 11 | Cldn11 | nm_008770 | gctccaagggcctgtgggc | tgtcaacagcagcaagatggcg | Morita et al (1999) |
| Clusterin | Clu | nm_013492 | ccacgccatgaagattctcctgc | ctccctggacggcgttctga | Morales et al (1987) |
| Cyclin D2 | Ccnd2 | nm_009829 | ggaacctggccgcagtcacc | aatcatcgacggcgggtacatg | Tan et al (2005b) |
| Cystatin 12 | Cst12 | af440737 | ggatgacgattttgcctacaagttcct | ttctctctcctggaccttcctgca | Li et al (2002) |
| Cystatin 9 (Testatin) | Cst9 | nm_009979 | gatatttgcccctttcaggagagcc | agagaagtacgtgaccagtccatggg | Kanno et al (1999) |
| Desert hedgehog | Dhh | nm_007857 | ggcgcagaccgcctgatg | aaggcacggccttcgtagtgg | Bitgood et al (1996) |
| Espin | Espn | nm_019585 | gcttctggtcgggcattaccct | gtgtcatgccgtcttgggcg | Bartles et al (1996) |
| Follicle stimulating hormone receptor | Fshr | nm_013523 | ggccaggtcaacataccgcttg | tgccttgaaatagacttgttgcaaattg | Kliesch et al (1992) |
| Fyn proto-oncogene | Fyn | nm_008054 | gaagcggccctgtatggaaggtt | tgtgggcagggcatcctatagc | Maekawa et al (2002) |
| GATA binding protein 1 | Gata1 | nm_008089 | atggtcagaaccggcctctcatc | gagcttgaaatagaggccgcagg | Tan et al (2005a) |
| Inhibin beta-A | Inhba | bc053527 | catggagcagacctcggagatca | tggtcctggttctgttagccttgg | Kaipia et al (1992) |
| Inhibin beta-B | Inhbb | nm_008381 | gagcgcgtctccgagatcatca | cgtaccttcctcctgctgccctt | Kaipia et al (1992) |
| Interleukin 1alpha | Il1a | nm_010554 | ttggcgcttgagtcggcaaa | tcatgaagtgagccatagcttgcatc | Jonsson et al (1999) |
| Musashi homolog 1 | Msi1 | nm_008629 | tcactttcatggaccaggcgg | gttcacagacagccccccca | Saunders et al (2002) |
| Reproductive homeobox 5 | Rhox5 | nm_008818 | aggttcgcccagcatcgactg | gccgcagccctcctgatctt | Lindsey & Wilkinson (1996) |
| RIKEN 4930486L24 (Testin) | 4930486L 24Rik |
ay146988 | aaagacaatggcggcctcgc | ggccccactttagccactgcc | Cheng et al (1989) |
| Sex hormone binding globulin | Shbg | nm_011367 | gacattccccagcctcatgca | tgcctcggaagacagaaccacg | Wang et al (1989) |
| Spermatogenesis associated 2 | Spata2 | nm_170756 | gccgtgtgggcctgtgctt | ttccccaaatcaaacccaaggg | Graziotto et al (1999) |
| SRY-box containing gene 9 | Sox9 | nm_011448 | cgcggagctcagcaagactctg | tgtccgttcttcaccgacttcctc | Frojdman et al (2000) |
| Symplekin | sympk | xm_485873 | caagaagaagggccaagcatcga | aggaagttgtcaagcagggtggg | Keon et al (1996) |
| Tight junction protein 1 | Tjp1 | nm_009386 | gcggagagagacaagatgtccgc | ctctgaaaatgaggattatctcttccacca | Byers et al (1991) |
| Transferin | Trf | nm_133977 | caaatgcatcagcttccgtgacc | cggcatcgtacacccaaccc | Skinner & Griswold (1980) |
| Wilms tumor homolog | Wt1 | nm_144783 | gctccagctcagtgaaatggacagaa | ggccactccagatacacgccg | Mundlos et al (1993) |
| Wingless-related MMTV integration site 5A |
Wnt5a | nm_009524 | ctgcttctaccatgcgtttgctgg | gccatgggacagtgcggc | O'Shaughnessy et al (2007) |
| Leydig cell | |||||
| Cytochrome P450 side chain cleavage |
Cyp11a1 | nm_019779 | cacagacgcatcaagcagcaaaa | gcattgatgaaccgctgggc | O'Shaughnessy et al (2002) |
| 3B-hydroxysteroid dehydrogenase type 6 |
Hsd3b6 | nm_013821 | gctccagactgggactgctgacac | aatcctctggcccaaaaaccctc | O'Shaughnessy et al (2002) |
| Steroidogenic acute regulatory protein |
Star | nm_011485 | cgtcggagctctctgcttggttc | tcgtccccgttctcctgctg | O'Shaughnessy et al (2002) |
| Cytochrome P450 17alpha- hydroxylase |
Cyp17a1 | nm_007809 | tggtcccatctattctcttcgcctg | aggcgacgccttttccttgg | O'Shaughnessy et al (2002) |
| Luteinising hormone receptor | Lhr | nm_013582 | tcaggaatttgccgaagaaagaacag | gaagtcataatcgtaatcccagccactg | O'Shaughnessy et al (2002) |
| Platelet -derived growth factor A | Pdgfa | nm_008808 | gagcggctggctcgaagtcag | ctgcgaatgggcacaggcc | Gnessi et al (1995) |
| Germ cell | |||||
| Transition protein 1 | Tnp1 | nm_009407 | ggcgatgatgcaagtcgcaa | ccactctgataggatctttggcttttgg | Yelick et al (1989) |
| Sporulation protein meiosis-specific | Spo11 | nm_012046 | cgcgtggcctctagttctgagg | ggtatcatccgaaggccgacagaat | Shannon et al (1999) |
| Stimulated by retinoic acid gene 8 | Stra8 | nm_009292 | gaaggtgcatggttcaccgtgg | gctcgatggcgggcctgtg | Oulad-Abdelghani et al (1996) |
| Others | |||||
| Beta defensin 36 | def36 | nm_001037 247 |
tccccagtacgccacgaacg | ttgccgtggagattccagcatt | see text |
| Gata binding protein 4 | Gata4 | nm_008092 | cccttcgacagcccagtcctg | aggtagtgtcccgtcccatctcg | Ketola et al (1999) |
| Nuclear receptor subfamily 0b1 (Dax1) |
Nr0b1 | nm_007430 | cggaggctgggcacttgct | caatgtatttcacgcactgcaggc | Tamai et al. 1996 |
*
Reference describing localisation of mRNA transcripts in the testis