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Glycosidase mechanisms for hydrolysis. (a) ‘Classical’ mechanism for inversion of stereochemistry. (b) ‘Classical’ mechanism for retention of stereochemistry. (c) Substrate-assisted mechanism proposed for families 18, 20, 56, 84, 85 and possibly 25. (d) Mechanism using a tyrosine residue as the nucleophile proposed for families 33 and 34.
