Skip to main content
. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: J Neurosci. 2007 Nov 28;27(48):13210–13221. doi: 10.1523/JNEUROSCI.3056-07.2007

Figure 3.

Figure 3

Immunofluorescent staining of cultured mouse hippocampal neurons with the phospho-T840 GluR1 antibody. A, Confocal images showing that phospho-T840 GluR1-like immunofluorescence is present throughout the soma and MAP2-positive dendrites of hippocampal neurons. Scale bar, 50 μm. B, Higher magnification images of processes in cultured neurons reveal extensive colocalization of phospho-T840 GluR1-like immunofluorescence with the postsynaptic marker PSD-95 (top) but little overlap with the presynaptic marker synaptophysin (bottom). Scale bar, 5μm. C, Triple staining of cultured hippocampal neurons with anti-phospho-T840 (green), anti-GluR1 (red), and anti-MAP2 (blue) antibodies reveals strong colocalization of phospho-T840 GluR1-like immunofluorescence with total GluR1 staining. Scale bar, 2 μm. D, Cultures of hippocampal neurons obtained from wild-type and GluR1-null mutant mice were stained with anti-MAP2 (blue) and anti-synaptophysin (green) antibodies. Immunostaining for both GluR1 (red; top two panels) and phospho-T840 GluR1 (red; bottom two panels) was strongly diminished in GluR1 -/- neurons. Scale bar, 2 μm.