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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: J Neurosci. 2007 Nov 28;27(48):13210–13221. doi: 10.1523/JNEUROSCI.3056-07.2007

Figure 4.

Figure 4

GluR1 phosphorylation at T840 is not enhanced by LTP-inducing patterns of synaptic stimulation. A, A TPS protocol consisting of 150 pulses of presynaptic fiber stimulation delivered at 5 Hz induces significant LTP in hippocampal CA1 mini-slices. Field EPSPs were potentiated to 161 ± 8% of baseline 60 min post-TPS (p < 0.001 compared with baseline; n = 5). The inset shows fEPSPs recorded during baseline and 45 min post-TPS. Calibration: 2 mV, 5 ms. B, LTP inducing patterns of synaptic stimulation have no effect on GluR1 phosphorylation at T840. CA1 mini-slices obtained from the same animal were either unstimulated (US) or snap frozen 5 min after activating presynaptic fibers in stratum radiatum with a TPS protocol (150 pulses at 5 Hz). Whereas TPS induced a significant increase in GluR1 phosphorylation at S831 (*p < 0.01 compared with US controls), it had no effect on GluR1 phosphorylation at T840 or S845 (n = 8 experiments). The immunoblots show phospho-GluR1 and total-GluR1 levels in samples from one experiment. Error bars indicate SEM.