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The vanadate-sensitive ATP hydrolysis was measured in PMFs prepared from PGs incubated in hypo- (198 mM), iso- (298 mM), and hyperosmolar (398 mM mannitol) germination medium. The specific activity is given in nmol mg−1 min−1.
| Mannitol Concentration | Specific Activity | Osmotic Treatment |
|---|---|---|
| mM | nmol Pi mg−1 min−1 | |
| 198 | 89.50 ± 19.92 | Hypo |
| 298 | 130.20 ± 7.35 | Iso |
| 398 | 164.29 ± 30.30 | Hyper |
Mean values ± sd (n = 3).
