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. Author manuscript; available in PMC: 2011 May 27.
Published in final edited form as: Mol Ther. 2011 Jan 11;19(4):642–649. doi: 10.1038/mt.2010.293

Figure 1.

Figure 1

Co-transduction of AAVs in wild type retinas.

Eyes of wild type mice were subretinally injected with a mixture of 1.5×109 vp AAV-EGFP and 1.5×109 vp AAV-DsRed. Two weeks post-injection eyes were fixed (n=5), whole mounted for imaging, then cryosectioned (12 μm) and processed for histology. Nuclei were counterstained with DAPI. a, b and c: representative whole mounts illustrating EGFP (a), DsRed (b) and overlay of EGFP and DsRed signals (c). Representative sections demonstrate significant co-expression (f) of EGFP (d) and DsRed (e) signals at the cellular level in the outer nuclear layer. In order to obtain a clearer view of the markers the DAPI (blue) signal was edited out from the ONL. RPE: retinal pigment epithelium; ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer. Scale bars: 1 mm (a, b and c) and 25 μm (d, e and f).