Figure 6.
MGE-derived cells from Robo1−/− mutants do not respond to Sema3A or Sema3F. (A-D) Migration of cells away from E13.5 MGE explants prepared from Robo1+/− (A,C) and Robo1−/− (B,D) littermates treated with control conditioned media (CM-myc; A,B) or CM-Sema3A (C,D). (E,F) Quantification of migration from Robo1+/− and Robo1−/− MGE explants treated with Sema3A (E) or Sema3F (F). (G,H) Quantification of the number of dissociated MGE cells from Robo1+/− and Robo1−/− mice used in a Boyden’s chamber assay to test their response to Sema3A (G) or Sema3F (H). Scale bar: 100 μm. **p<0.01; ***p<0.001.
