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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Neurosci. 2011 Apr 20;31(16):6174–6187. doi: 10.1523/JNEUROSCI.5464-10.2011

Figure 7.

Figure 7

GN11 cells express Robo receptors and respond to class 3 semaphorins and Slit1. (A) RT-PCR analysis showed expression of Robo1-3 in GN11 cells. (B) Immunohistochemistry confirmed the expression of Robo receptors in GN11 cells. (C,D) Quantification of GN11 cell migration in a Boyden’s chamber, containing Sema3A, Sema3F, Slit1 or control (CM-myc) conditioned media (C). (D) Robo1-DN transfected GN11 cells are less responsive to Sema3A than control (GFP) transfected cells. Scale bar: 100 μm. **p<0.01; ***p<0.001.