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Inhibition of ligand binding by CHIPS. Cells transfected with C5a receptor (C5aR), C5L2 or formyl peptide receptor (FPR) were co-incubated with the stated concentrations of rCHIPS28-149 with His6-C5a (50 nM), His6-C5a des Arg (50 nM) or FITC-formyl peptide (500 nM). Data shown are the means of three separate experiments performed in duplicate, ±S.E.M.
