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Kinetic analysis of ligand binding to chemoattractant receptors. The kinetics of FITC-rCHIPS28-149 binding to cells transfected with C5a receptors was measured using flow cytometry after addition of the fluorescent ligand to RBL cells transfected with C5aR at 300 nM. Dissociation was initiated by the addition of 14 μM unlabelled ligand (arrow): Data are shown as geometric means of fluorescence values obtained in 0.1 s time windows and is one experiment, typical of five performed.
