Figure 7. NΔ390 dominant negative cadherin expression results in a similar phenotype to γ–catenin(L127A) expression in disruption of LMC divisional segregation.
A-C. GFP, Islet-1 and HB9 immunoreactivity following NΔ390 cadherin expression shows stalled motor neurons and perturbed divisional segregation of the LMC.
D-F. Hb9 and Islet-1 immunoreactivity on the contralateral LMC.
Dotted lines in A-F show the ventricular surface.
Lines in C and F show the medio-lateral extent of the LMC-note that following N Δ390 cadherin expression the LMC spans approximately twice the extent of the contralateral LMC.
G. Percentage of Hb9 and Islet-1 cells adjacent to the ventricular zone following N Δ390 cadherin expression. Contralateral spinal cords have zero cells in similar positions.
H. Divisional mixing index following NΔ390 cadherin expression compared to contralateral spinal cord (student’s t-test of bins 0, 1 and 2, p<0.001. χ2 p<0.001 of the entire distribution 2df). Error bars in G, H indicate SEM.
I-K. Pax6 expression following NΔ390 cadherin expression, marked by GFP in I. K is a magnified area illustrating the buckled Pax 6 domain (shown by the arrow) in J.
L-N. Transitin immunoreactivity (M, N) following NΔ390 cadherin expression marked by GFP in L. N is a magnified region of M, shown by the black lines. Arrowheads in N show transitin fibres coursing from the ventricle surface to the pial surface of the spinal cord.