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. Author manuscript; available in PMC: 2009 Apr 20.
Published in final edited form as: Mol Endocrinol. 2009 Jan 15;23(4):529–538. doi: 10.1210/me.2008-0399

Figure 3.

Figure 3

Molecular characterisation of BAC PRL-Luc and PRL-d2eGFP transgenic rats. a) FISH analysis of metaphase and interphase nuclei of transgenic rat spleenocytes. Left panels PRL-Luciferase line 49; right panel PRL-d2eGFP line 455 b and c) Fluorescent immuno cytochemistry of female (left) and male (right) pituitary sections. Green fluorescence: luciferase b) or d2eGFP c), red fluorescence: prolactin. Scale bar, 20 μm; d) Real-time qPCR of luciferase, d2eGFP and rat prolactin in the pituitary gland of female and male transgenic rats. (*p<0.05 rat Prl vs luciferase in males and females; n=5 female; n=4 male; * p<0.05 rat Prl vs d2eGFP in males; n=3 female; n=4 male)