Figure 5.
Extra-pituitary expression of luciferase in BAC PRL-Luc transgenic rat. a) In vivo imaging of vehicle (V) and LPS-treated rats. The rats were injected i.p (arrows indicate site of injection) either with physiological saline or with 3mg/ml LPS and imaged 16 h after the treatment. The images were acquired with an IVIS Spectrum (Xenogen/ Caliper): 30s integration time, Bin (HR) 8, FOV 19.6, f1 b) RT-PCR strategy for the analysis of luciferase expression driven by the extra-pituitary promoter. Dotted line: observed alternative splicing c) RT-PCR result. M: 100bp ladder T: thymus, SP: spleen.
