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. Author manuscript; available in PMC: 2012 Mar 22.
Published in final edited form as: Front Immunol. 2012 Mar;3(March):1–16. doi: 10.3389/fimmu.2012.00019

FIGURE 1. Expression of epidermal differentiation markers in thymi and skin of WT mice.

FIGURE 1

Thymi or skin were collected from WT mice and stained for four epidermal markers, CK6, CK10, LEKTI, and involucrin by immunohistochemistry. CK6 showed intense reaction with the basal layer, the spinous layer, and the granular layer of skin epidermis. In the thymus, CK6 stained the outer layers of HCs and some staining in the medullary thymic epithelial-like cells. CK10 showed intense reaction with the stratum spinous layer, the granular layer, and the cornified layer of skin epidermis. In the thymus, CK10 strongly stained the outer layers of HCs in the medulla and gave also weaker staining in the central portion of HCs as well as in some medullary thymic epithelial-like cells. LEKTI stained the granular layer of skin epidermis and localized in the outer layers of HCs in the thymus. Involucrin mainly stained the granular layer, and also gave minimal staining in the cornified layer of skin epidermis. In the thymus, involucrin stained the outer layer of HCs. Shown are representative stainings of at least three experiments, bars correspond to 40 μm.