Skip to main content
. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Invest Dermatol. 2012 Jun 14;132(12):2691–2699. doi: 10.1038/jid.2012.201

Figure 5. KGF inhibits differentiation of epithelial cells.

Figure 5

A) Addition of KGF to keratinocyte cultures inhibited calcium induced differentiation as assessed by Western blotting of involucrin, keratin-1 and transglutaminase levels. B) Organotypic cultures grown in the presence of 2 or 10 ng/mL KGF showed disrupted differentiation. Representative cultures from triplicate analyses are shown. C) Knockdown of KGF levels in Rb-depleted fibroblasts was assessed by Q-PCR. D) Organotypic cultures using fibroblasts in C showed that KGF depletion did not significantly alter differentiation in control cultures (compare a-c with d-f and g-i), however in cultures grown with Rb-depleted fibroblasts, KGF depletion restored epithelial differentiation (compare j-l with m-o and p-r). E) Brdu incorporation in the epithelium of cultures shown in D. Scale bars represent 100 μM.