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. Author manuscript; available in PMC: 2006 Jan 26.
Published in final edited form as: J Cell Biol. 2005 Jul 11;170(2):183–190. doi: 10.1083/jcb.200504167

Figure 4. TIRFM.

Figure 4

(A) GFP-CAPRI translocation induced by 100 μM histamine monitored by TIRFM (representative experiment for n = 4 cells). (B) GFP-RASAL translocation induced by 100 μM histamine monitored by TIRFM (representative experiment for n = 3 cells). (C) Representative experiment of GFP-CAPRI translocation in HeLa cells stimulated with 1 μM histamine (n = 3 cells). Arrows indicate mini-oscillations in one trace; similar oscillations are also detectable in other cells as shown. (D) Representative trace of sequential Fura-2 (black trace; single wavelength 380-nm excitation) and TIRF imaging of GFP-CAPRI (blue trace). Mini-oscillations correlate with cytosolic Ca2+ spikes.